Western Blot: Difference between revisions
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====Induction and Overnight Incubation of Bacterial Culture==== | ====Induction and Overnight Incubation of Bacterial Culture==== | ||
===Day 2=== | ===<u>Day 2</u>=== | ||
====Obtaining Supernatant and Lysate Samples from Bacterial Culture==== | ====Obtaining Supernatant and Lysate Samples from Bacterial Culture==== | ||
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====Transferring the SDS-PAGE Gel to the Membrane==== | ====Transferring the SDS-PAGE Gel to the Membrane==== | ||
===Day 3=== | ===<u>Day 3</u>=== | ||
====Primary Antibody Staining (Overnight)==== | ====Primary Antibody Staining (Overnight)==== | ||
===Day 4=== | ===<u>Day 4</u>=== | ||
====Secondary Antibody Staining==== | ====Secondary Antibody Staining==== | ||
====Imaging the Blot==== | ====Imaging the Blot==== | ||
Revision as of 21:44, 11 May 2023
Goal
To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host.