Western Blot: Difference between revisions
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==Protocol== | ==Protocol== | ||
===Day 1=== | ===<u>Day 1</u>=== | ||
====Induction and Overnight Incubation of Bacterial Culture==== | ====Induction and Overnight Incubation of Bacterial Culture==== | ||
Revision as of 21:43, 11 May 2023
Goal
To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host.