Western Blot: Difference between revisions
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====Running an SDS-PAGE on Supernatant/Lysate Samples==== | ====Running an SDS-PAGE on Supernatant/Lysate Samples==== | ||
====Transferring the SDS-PAGE | ====Transferring the SDS-PAGE Gel to the Membrane==== | ||
===Day 3=== | ===Day 3=== | ||
==== ==== | ====Primary Antibody Staining (Overnight)==== | ||
===Day 4=== | ===Day 4=== | ||
==== | ====Secondary Antibody Staining==== | ||
====Imaging the Blot==== | |||
Revision as of 21:41, 11 May 2023
Goal
To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host.