Western Blot: Difference between revisions
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(Created page with "==Goal== * To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host. ==Protocol== ===Day 1=== ====Induction and Overnight Incubation of Bacterial Culture==== '''Day 2: Obtaining Supernatant and Lysate Samples from Bacterial Culture''' '''Da...") |
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====Induction and Overnight Incubation of Bacterial Culture==== | ====Induction and Overnight Incubation of Bacterial Culture==== | ||
===Day 2=== | |||
====Obtaining Supernatant and Lysate Samples from Bacterial Culture==== | |||
====Running an SDS-PAGE on Supernatant/Lysate Samples==== | |||
====Transferring the SDS-PAGE gel to the Membrane==== | |||
===Day 3=== | |||
==== ==== | |||
===Day 4=== | |||
====Induction and Overnight Incubation of Bacterial Culture==== | |||
Revision as of 21:39, 11 May 2023
Goal
To extract protein samples from the bacterial host, run them through an SDS-PAGE gel, and use antibodies to detect specific proteins in the size-separated protein samples. This technique is useful for confirming the presence of a particular protein inside or outside of the bacterial host.