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<strong>MediaWiki has been installed.</strong>
=== [[Lab Floor Plan (with list of materials)]] ===


== General microbiology protocols ==
===[[Media Recipes]]===
===[[Reagent Recipes]]===
===[[Working with Antibiotics]]===
===[[Freezing -80 Stocks]]===
===[[Freezing Aliquots]]===
===[[Competition Assays]]===
===[[Generic PCR]]===
===[[Gradient PCR]]===
===[[Running DNA Gels]]===
===[[Protein Purification]]===
===[[Protein Sample Concentration]]===
== Cloning and gene manipulation ==
===[[Commonly Used Plasmids]]===
===[[Plasmid Purification]]===
===[[Digest and Ligation]]===
===[[Gel Purification]]===
===[[Creating Competent E. coli Cells]]===
===[[Transformation]]===
===[[Transformation — non-competent E. coli]]===
===[[Gibson Assembly]]===
===[[Qubit dsDNA Broad Range Assay]]===
===[[Preparing Sanger Sequencing (Eurofins)]]===
===[[Preparing Plasmid Sequencing (plasmidsaurus)]]===
===[[Creating Lac- E. coli Mutants]]===
== ''Arabidopsis thaliana'' protocols ==
===[[Creating Sterile Agar Plates]]===
===[[Sterile Seeding Protocol]]===
===[[Germination Protocol for ''Arabidopsis thaliana'' Seeds in Non-Sterile Experiments]]===
===[[Growth Stage Phenotype Definitions]]===
===[[Growth Conditions for ''Arabidopsis thaliana'']]===
===[[Measuring Light with HOBO Data Loggers]]===
===[[Inoculation of ''Arabidopsis thaliana'' with Microbes]]===
===[[Removal and DNA Extraction of Phyllosphere Microbes]]===
===[[ARISA]]===
===[[Measuring ''A. thaliana'' Phenotype using FIJI by Hand]]===
===[[DNeasy PowerSoil Protocol]]===
===[[Fiji Measurement]]===
===[[Making Boxes]]===
===[[Growing ''A. thaliana'' for Seed Harvest]]===
===[[Growing ''A. thaliana'' in Cut Pipet Tips]]===
== ''Streptococcus pneumoniae'' protocols ==
===[[Dual Layer Assays]]===
===[[Streptococcus DNA Extraction]]===
===[[Streptococcus CRISPR-Cas9 Editing]]===
===[[Streptococcus Transformation]]===
===[[Streptococcus Growth Curve Protocol]]===
===[[Streptococcus Growth Curve and Cell Count in Liquid Media]]===
===[[Log Phase Growth Curve and Cell Count in Liquid Media]]===
===[[Streptococcus Bacteriocin (Dual Layer) Assays - Original]]===
===[[Streptococcus Bacteriocin (Dual Layer) Assays - Early Producer]]===
===[[Streptococcus Bacteriocin (Dual Layer) Assays - Light and Normal Target Lawns]]===
===[[Streptococcus Bacteriocin (Dual Layer) Assays - Finding Producer-Resistant Target Bacteria]]===
===[[Streptococcus Bacteriocin (Dual Layer) Assays - Finding Producer-Resistant Target Bacteria (6-well plate version)]]===
== ''Streptococcus suis'' protocols ==
===[[Streptococcus suis Transformation]]===
===[[Measuring Absorbance in Streptococcus]]===
===[[Streptococcus DNA Extraction]]===
===[[Streptococcus Competence Induction]]===
===[[Peptide Synthesis]]===
===[[Peptide Cleavage]]===
===[[Mass Spectrometery]]===
===[[Plate Reader Assay and Growth Curve]]===
===[[Measuring Competence : Fixation and Flow Cytometry]]===
== ''Streptococcus mutans'' protocols ==
===[[Streptococcus mutans Growth]]===
===[[Streptococcus mutans Transformation]]===
== [[Interactions Protocols]] ==
===[[Zone of Inhibition Assay]]===
== [[Remote Molecular Biology]] ==
== [[Effect of Laboratory Protocols on Student Learning]] ==
== Interesting Podcasts to Listen to When Doing Lab Work! ==
This Week in Microbiology
By Vincent Racaniello
5 stars! Amazing podcast to learn all about different types of microbiology research! This podcast goes pretty in depth into different current scientific papers so it is a great way to learn about current research.
This Week in Virology
By Vincent Racaniello
Ologies
By Alie Ward
5 stars! Great all around podcast made for a more general audience. There are many different episodes on scientific topics including Environmental Microbiology, Mathematical Biology, and much much more. This is a great podcast to explore different careers and listen to some amazing speakers.
Overheard at National Geographic
By National Geographic
4 stars! Great podcast to hear about the many wonders of the world. It also has shorter episodes and is a great way to learn about the world.
Journey to the Micro Cosmos
3 stars! Short 10 minute episodes about cool microbes.
== [[Waste Disposal]] ==
== Cambridge protocols ==
=== [[Storage buffer]] ===
=== [[transformation of R5(2)-mCh-FL-BST and ]] ===
=== [[expression]] ===
=== [[lysis and immobilization]] ===
== Detailed Lab Task Descriptions ==
=== [[Serological Pipetes]] ===
=== [[Media]] ===
=== [[Medical Waste]] ===
=== [[Tips/Tubes]] ===
=== [[Glassware (no 50 mL flasks)]] ===
=== [[Just Flasks]] ===
=== [[Water/Ethanol]] ===
=== [[Balance/Microwave/Gel Box/Virkon]] ===
==[[Bio320 Microbe Species Wikipedia Pages]]==
== Getting started with MediaWiki ==
Consult the [https://www.mediawiki.org/wiki/Special:MyLanguage/Help:Contents User's Guide] for information on using the wiki software.
Consult the [https://www.mediawiki.org/wiki/Special:MyLanguage/Help:Contents User's Guide] for information on using the wiki software.
== Getting started ==
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Configuration_settings Configuration settings list]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Configuration_settings Configuration settings list]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:FAQ MediaWiki FAQ]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:FAQ MediaWiki FAQ]
* [https://lists.wikimedia.org/postorius/lists/mediawiki-announce.lists.wikimedia.org/ MediaWiki release mailing list]
* [https://lists.wikimedia.org/mailman/listinfo/mediawiki-announce MediaWiki release mailing list]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Localisation#Translation_resources Localise MediaWiki for your language]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Localisation#Translation_resources Localise MediaWiki for your language]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Combating_spam Learn how to combat spam on your wiki]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Combating_spam Learn how to combat spam on your wiki]

Revision as of 13:10, 16 December 2022

Lab Floor Plan (with list of materials)

General microbiology protocols

Media Recipes

Reagent Recipes

Working with Antibiotics

Freezing -80 Stocks

Freezing Aliquots

Competition Assays

Generic PCR

Gradient PCR

Running DNA Gels

Protein Purification

Protein Sample Concentration

Cloning and gene manipulation

Commonly Used Plasmids

Plasmid Purification

Digest and Ligation

Gel Purification

Creating Competent E. coli Cells

Transformation

Transformation — non-competent E. coli

Gibson Assembly

Qubit dsDNA Broad Range Assay

Preparing Sanger Sequencing (Eurofins)

Preparing Plasmid Sequencing (plasmidsaurus)

Creating Lac- E. coli Mutants

Arabidopsis thaliana protocols

Creating Sterile Agar Plates

Sterile Seeding Protocol

Germination Protocol for ''Arabidopsis thaliana'' Seeds in Non-Sterile Experiments

Growth Stage Phenotype Definitions

Growth Conditions for ''Arabidopsis thaliana''

Measuring Light with HOBO Data Loggers

Inoculation of ''Arabidopsis thaliana'' with Microbes

Removal and DNA Extraction of Phyllosphere Microbes

ARISA

Measuring ''A. thaliana'' Phenotype using FIJI by Hand

DNeasy PowerSoil Protocol

Fiji Measurement

Making Boxes

Growing ''A. thaliana'' for Seed Harvest

Growing ''A. thaliana'' in Cut Pipet Tips

Streptococcus pneumoniae protocols

Dual Layer Assays

Streptococcus DNA Extraction

Streptococcus CRISPR-Cas9 Editing

Streptococcus Transformation

Streptococcus Growth Curve Protocol

Streptococcus Growth Curve and Cell Count in Liquid Media

Log Phase Growth Curve and Cell Count in Liquid Media

Streptococcus Bacteriocin (Dual Layer) Assays - Original

Streptococcus Bacteriocin (Dual Layer) Assays - Early Producer

Streptococcus Bacteriocin (Dual Layer) Assays - Light and Normal Target Lawns

Streptococcus Bacteriocin (Dual Layer) Assays - Finding Producer-Resistant Target Bacteria

Streptococcus Bacteriocin (Dual Layer) Assays - Finding Producer-Resistant Target Bacteria (6-well plate version)

Streptococcus suis protocols

Streptococcus suis Transformation

Measuring Absorbance in Streptococcus

Streptococcus DNA Extraction

Streptococcus Competence Induction

Peptide Synthesis

Peptide Cleavage

Mass Spectrometery

Plate Reader Assay and Growth Curve

Measuring Competence : Fixation and Flow Cytometry

Streptococcus mutans protocols

Streptococcus mutans Growth

Streptococcus mutans Transformation

Interactions Protocols

Zone of Inhibition Assay

Remote Molecular Biology

Effect of Laboratory Protocols on Student Learning

Interesting Podcasts to Listen to When Doing Lab Work!

This Week in Microbiology

By Vincent Racaniello

5 stars! Amazing podcast to learn all about different types of microbiology research! This podcast goes pretty in depth into different current scientific papers so it is a great way to learn about current research.

This Week in Virology By Vincent Racaniello


Ologies By Alie Ward 5 stars! Great all around podcast made for a more general audience. There are many different episodes on scientific topics including Environmental Microbiology, Mathematical Biology, and much much more. This is a great podcast to explore different careers and listen to some amazing speakers.

Overheard at National Geographic By National Geographic

4 stars! Great podcast to hear about the many wonders of the world. It also has shorter episodes and is a great way to learn about the world.

Journey to the Micro Cosmos 3 stars! Short 10 minute episodes about cool microbes.

Waste Disposal

Cambridge protocols

Storage buffer

transformation of R5(2)-mCh-FL-BST and

expression

lysis and immobilization

Detailed Lab Task Descriptions

Serological Pipetes

Media

Medical Waste

Tips/Tubes

Glassware (no 50 mL flasks)

Just Flasks

Water/Ethanol

Balance/Microwave/Gel Box/Virkon

Bio320 Microbe Species Wikipedia Pages

Getting started with MediaWiki

Consult the User's Guide for information on using the wiki software.