Storage buffer: Difference between revisions
Jump to navigation
Jump to search
PreDec2022>MadeleineGallic (Created page with "== Storage Buffer Preparation for R5(2)-mCh-FL-BST == ==== final concentration (with pH 7.1): ==== {| class="wikitable" border="1" |+ | storage buffer recipe |- ! Amount !...") |
m (1 revision imported) |
(No difference)
|
Latest revision as of 12:57, 16 December 2022
Storage Buffer Preparation for R5(2)-mCh-FL-BST
final concentration (with pH 7.1):
storage buffer recipe | |
Amount | Chemical |
---|---|
30 mM | KCl |
2.5 mM | TRIS-HCl |
0.025 mM | EDTA |
1 mM | DTT |
0.1% | Triton X-100 |
procedure for 250 mL:
- weigh out the following powders: KCl, TRIS, EDTA, DTT
measured mass | |
Desired Amount | |
---|---|
KCL | 559.14 mg |
TRIS | 75.71 mg |
EDTA | 1.83 mg |
DTT | 38.56 mg |
- add all chemicals directly to a 250 mL pyrex container
- add 250 mL H2O to container
- add 250 μL Triton X-100
- adjust pH to 7.25
Calculations:
Quantity calculator | ||||
g/mol | desired mM | desired mL | required mg | |
---|---|---|---|---|
KCL | 74.5513 | 30 | 250 | 559.13475 |
TRIS | 121.14 | 2.5 | 250 | 75.7125 |
EDTA | 292.24 | 0.025 | 250 | 1.8265 |
DTT | 154.253 | 1 | 250 | 38.56325 |