Remote Molecular Biology: Difference between revisions
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PreDec2022>Jharna Jahnavi No edit summary |
PreDec2022>EricMiller |
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==[[PCR-based Methods]]== | ==[[PCR-based Methods]]== | ||
Loop-mediated isothermal amplification (LAMP): | |||
*[https://link.springer.com/content/pdf/10.1007%2Fs12275-015-4656-9.pdf Minireview: Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects] | |||
*[https://www.nature.com/articles/nmeth.2520.pdf Simultaneous DNA amplification and detection using a pH-sensing semiconductor system] | |||
*[https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000865&type=printable LAMP for Human African Trypanosomiasis: A Comparative Study of Detection Formats] | |||
*[https://www.future-science.com/doi/pdf/10.2144/000114253 Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes] | |||
qPCR machines: | |||
*open PCR machine was bought by Chai; it is no longer commercially made [https://openpcr.org/]. This website does have the maker instructions for it. | |||
*Chai makes an open qPCR machine [https://www.chaibio.com/openqpcr]. $4,300 for one channel, $6,000 for dual channel. [https://github.com/chaibio/chaipcr open source code] [https://grabcad.com/library/chai-open-qpcr-1 open source construction] | |||
* Biomeme has a pre-shipment travel qPCR machine with 3 channels. $9,950. [https://shop.biomeme.com/collections/devices/products/three9-real-time-pcr-thermocycler-pre-order?variant=656540172300] | |||
==[[Culture-based Methods]]== | ==[[Culture-based Methods]]== |
Revision as of 18:16, 26 February 2019
Topics to Investigate
Articles / Papers to read
Relevant Pathogens
General Lab Methods
PCR-based Methods
Loop-mediated isothermal amplification (LAMP):
- Minireview: Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects
- Simultaneous DNA amplification and detection using a pH-sensing semiconductor system
- LAMP for Human African Trypanosomiasis: A Comparative Study of Detection Formats
- Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
qPCR machines:
- open PCR machine was bought by Chai; it is no longer commercially made [1]. This website does have the maker instructions for it.
- Chai makes an open qPCR machine [2]. $4,300 for one channel, $6,000 for dual channel. open source code open source construction
- Biomeme has a pre-shipment travel qPCR machine with 3 channels. $9,950. [3]