Reagent Recipes: Difference between revisions
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PreDec2022>Yhahn |
PreDec2022>EricMiller No edit summary |
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| Line 34: | Line 34: | ||
For 1 liter: | For 1 liter: | ||
*NaCl 80g | |||
*KCl 2g | |||
*Na<sub>2</sub>HPO<sub>4</sub> 14.4g | |||
*KH<sub>2</sub>PO<sub>4</sub> 2.4g | |||
Stir salts with MilliQ water. This may take a significant time to dissolve! | Stir salts with MilliQ water. This may take a significant time to dissolve! | ||
| Line 47: | Line 46: | ||
=Tris Buffer= | =Tris Buffer= | ||
For Tris buffer at pH 8.0 (at 25 degrees) | For Tris buffer at pH 8.0 (at 25 degrees) | ||
*4.44 g/L Tris HCl | |||
*2.65 g/L Tris Base | |||
'''Do not adjust pH or check the pH with the MEE lab pH probe. This will destroy the probe!''' | '''Do not adjust pH or check the pH with the MEE lab pH probe. This will destroy the probe!''' | ||
Revision as of 15:20, 11 March 2019
Reagent Information
| Reagent | Abbreviation | Stock Solution
Concentration |
Solvent | Stock Concentration to
Working Concentration |
Notes |
|---|---|---|---|---|---|
| Bromo-chloro-indolyl-galactopyranoside | X-gal | 20mg/ml | Dimethylformamide (DMF) | 250x | Use DMF in a chemical hood |
| Isopropyl β-D-1-thiogalactopyranoside | IPTG | 0.1M | H2O | 1000x | Light sensitive. Molecular weight: 238.31 g/mol |
Glycerol for freezing aliquots
We use sterile 80% glycerol in water. We dilute this 1:3 (400ul 80% glycerol; 1200ul culture) for creating freezer stocks with a final glycerol concentration of 20%.
10x PBS
Used to create 1x PBS for dilution or washing cells of any species.
For 1 liter:
- NaCl 80g
- KCl 2g
- Na2HPO4 14.4g
- KH2PO4 2.4g
Stir salts with MilliQ water. This may take a significant time to dissolve!
Adjust pH with NaOH to 7.4. This will take a significant amount of NaOH; add in 200ul increments.
Tris Buffer
For Tris buffer at pH 8.0 (at 25 degrees)
- 4.44 g/L Tris HCl
- 2.65 g/L Tris Base
Do not adjust pH or check the pH with the MEE lab pH probe. This will destroy the probe!