Reagent Recipes: Difference between revisions
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PreDec2022>EricMiller (Created page with "=Reagent Information= {| class="wikitable" |- ! Reagent ! Abbreviation ! Stock Solution Concentration ! Solvent ! Stock Concentration to Working Concentration ! Notes |- |...") |
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=Glycerol for freezing aliquots= | =Glycerol for freezing aliquots= | ||
We use sterile 80% glycerol in water. We dilute this 1:3 (400ul 80% glycerol; 1200ul culture) for creating freezer stocks with a final glycerol concentration of 20%. | We use sterile 80% glycerol in water. We dilute this 1:3 (400ul 80% glycerol; 1200ul culture) for creating freezer stocks with a final glycerol concentration of 20%. | ||
=10x PBS= | |||
Used to create 1x PBS for dilution or washing cells of any species. | |||
For 1 liter: | |||
**NaCl 80g | |||
**KCl 2g | |||
**Na<sub>2</sub>HPO<sub>4</sub> 14.4g | |||
**KH<sub>2</sub>PO<sub>4</sub> 2.4g | |||
Stir salts with MilliQ water. This may take a significant time to dissolve! | |||
Adjust pH with NaOH to 7.4. This will take a significant amount of NaOH; add in 200ul increments. | |||
Revision as of 17:28, 30 November 2018
Reagent Information
| Reagent | Abbreviation | Stock Solution
Concentration |
Solvent | Stock Concentration to
Working Concentration |
Notes |
|---|---|---|---|---|---|
| Bromo-chloro-indolyl-galactopyranoside | X-gal | 40mg/ml | Dimethylformamide (DMF) | 500x | Use DMF in a chemical hood |
| Isopropyl β-D-1-thiogalactopyranoside | IPTG | 0.1M | H2O | 1000x | Light sensitive. Molecular weight: 238.31 g/mol |
Glycerol for freezing aliquots
We use sterile 80% glycerol in water. We dilute this 1:3 (400ul 80% glycerol; 1200ul culture) for creating freezer stocks with a final glycerol concentration of 20%.
10x PBS
Used to create 1x PBS for dilution or washing cells of any species.
For 1 liter:
- NaCl 80g
- KCl 2g
- Na2HPO4 14.4g
- KH2PO4 2.4g
Stir salts with MilliQ water. This may take a significant time to dissolve!
Adjust pH with NaOH to 7.4. This will take a significant amount of NaOH; add in 200ul increments.