Peste Des Petits Ruminants: Difference between revisions
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PreDec2022>MadeleineGallic (Created page with "E Couacy-Hymann, F Roger, C Hurard, J.P Guillou, G Libeau, A Diallo. (2002). [https://www.sciencedirect.com/science/article/pii/S016609340100386X Rapid and sensitive detection...") |
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*this assay was based on rapid purification of RNA on glass beads followed by RT-PCR. They used a set of primers created to target NP3/NP4 that encodes for the nucleocapsidase protein. The PCR was then detected using UV illumination after agarose gel electrophoresis or by hybridization using a oligonucleotide probe after blot transfer. They found that this technique was 1000-fold more sensitive than convention titration techniques. | *this assay was based on rapid purification of RNA on glass beads followed by RT-PCR. They used a set of primers created to target NP3/NP4 that encodes for the nucleocapsidase protein. The PCR was then detected using UV illumination after agarose gel electrophoresis or by hybridization using a oligonucleotide probe after blot transfer. They found that this technique was 1000-fold more sensitive than convention titration techniques. | ||
C.Senthil, KumaraG.Dhinakar, RajaA.Thangaveluab, M.S.Shaila. (2007) [Performance of RT-PCR-ELISA for the detection of peste des petits ruminants virus https://www.sciencedirect.com/science/article/pii/S0921448806002458] | |||
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Revision as of 08:54, 9 April 2019
E Couacy-Hymann, F Roger, C Hurard, J.P Guillou, G Libeau, A Diallo. (2002). Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay need access.
- this assay was based on rapid purification of RNA on glass beads followed by RT-PCR. They used a set of primers created to target NP3/NP4 that encodes for the nucleocapsidase protein. The PCR was then detected using UV illumination after agarose gel electrophoresis or by hybridization using a oligonucleotide probe after blot transfer. They found that this technique was 1000-fold more sensitive than convention titration techniques.
C.Senthil, KumaraG.Dhinakar, RajaA.Thangaveluab, M.S.Shaila. (2007) [Performance of RT-PCR-ELISA for the detection of peste des petits ruminants virus https://www.sciencedirect.com/science/article/pii/S0921448806002458]