Peptide Synthesis: Difference between revisions
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'''Day 1: Weighing Out Peptides''' | |||
1. Take A.A from the fridge to let them warm (''This prevents water from condensing into containers which may disrupt the reaction). | |||
2. While warming, set up clean cartridges in a metal rack. To reduce deletion sequences and improve peptide quality, make sure to double couple each peptide. | |||
3. Weigh out each amino acid (A.A) on the scale based on previous calculations for the 0.5 mmol reaction. Pour into labeled cartridge. | |||
4. Parafilm cartridges and place back into the fridge. | |||
* Time permitting, skip step 4 and add HBTU to the cartidges (refer to ''Day 2 Protocol'') | |||
'''Day 2: HBTU and Synthesis''' | |||
Revision as of 20:05, 5 April 2019
Day 1: Weighing Out Peptides
1. Take A.A from the fridge to let them warm (This prevents water from condensing into containers which may disrupt the reaction).
2. While warming, set up clean cartridges in a metal rack. To reduce deletion sequences and improve peptide quality, make sure to double couple each peptide.
3. Weigh out each amino acid (A.A) on the scale based on previous calculations for the 0.5 mmol reaction. Pour into labeled cartridge.
4. Parafilm cartridges and place back into the fridge.
- Time permitting, skip step 4 and add HBTU to the cartidges (refer to Day 2 Protocol)
Day 2: HBTU and Synthesis