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== [[Waste Disposal]] == | == [[Waste Disposal]] == | ||
=== | |||
==== Glassware ==== | |||
* Clean, disposable glassware (usually the test tubes) go into the blue/white cardboard box beside the BSL-2 hoods. | |||
* Contaminated (exposed to microbes) glassware goes into the '''red box''' under the BSL-2 hood closer to the spectrophotometer. Check with others for the location of the box if needed. '''DO NOT''' throw contaminated glassware into the other box, as it will cause a lot of trouble. | |||
== Cambridge protocols == | == Cambridge protocols == | ||
Revision as of 15:52, 17 April 2022
Lab Floor Plan (with list of materials)
General microbiology protocols
Media Recipes
Reagent Recipes
Working with Antibiotics
Freezing -80 Stocks
Freezing Aliquots
Competition Assays
Generic PCR
Gradient PCR
Running DNA Gels
Protein Purification
Protein Sample Concentration
Cloning and gene manipulation
Commonly Used Plasmids
Plasmid Purification
Digest and Ligation
Gel Purification
Creating Competent E. coli Cells
Transformation
Transformation — non-competent E. coli
Gibson Assembly
Creating Lac- E. coli Mutants
Arabidopsis thaliana protocols
Creating Sterile Agar Plates
Sterile Seeding Protocol
Germination Protocol for ''Arabidopsis thaliana'' Seeds in Non-Sterile Experiments
Growth Stage Phenotype Definitions
Growth Conditions for ''Arabidopsis thaliana''
Measuring Light with HOBO Data Loggers
Inoculation of ''Arabidopsis thaliana'' with Microbes
Removal and DNA Extraction of Phyllosphere Microbes
ARISA
Measuring ''A. thaliana'' Phenotype using FIJI by Hand
DNeasy PowerSoil Protocol
Fiji Measurement
Making Boxes
Growing ''A. thaliana'' for Seed Harvest
Growing ''A. thaliana'' in Cut Pipet Tips
Streptococcus pneumoniae protocols
Dual Layer Assays
Streptococcus DNA Extraction
Streptococcus Transformation
Streptococcus Growth Curve Protocol
Streptococcus Growth Curve and Cell Count in Liquid Media
Log Phase Growth Curve and Cell Count in Liquid Media
Streptococcus Bacteriocin (Dual Layer) Assays - Original
Streptococcus Bacteriocin (Dual Layer) Assays - Early Producer
Streptococcus Bacteriocin (Dual Layer) Assays - Light and Normal Target Lawns
Streptococcus Bacteriocin (Dual Layer) Assays - Finding Producer-Resistant Target Bacteria
Streptococcus Bacteriocin (Dual Layer) Assays - Finding Producer-Resistant Target Bacteria (6-well plate version)
Streptococcus suis protocols
Streptococcus suis Transformation
Measuring Absorbance in Streptococcus
Streptococcus DNA Extraction
Streptococcus Competence Induction
Peptide Synthesis
Peptide Cleavage
Mass Spectrometery
Plate Reader Assay and Growth Curve
Measuring Competence : Fixation and Flow Cytometry
Streptococcus mutans protocols
Streptococcus mutans Growth
Streptococcus mutans Transformation
Interactions Protocols
Zone of Inhibition Assay
Remote Molecular Biology
Effect of Laboratory Protocols on Student Learning
Interesting Podcats to Listen to When Doing Lab Work!
Waste Disposal
Glassware
- Clean, disposable glassware (usually the test tubes) go into the blue/white cardboard box beside the BSL-2 hoods.
- Contaminated (exposed to microbes) glassware goes into the red box under the BSL-2 hood closer to the spectrophotometer. Check with others for the location of the box if needed. DO NOT throw contaminated glassware into the other box, as it will cause a lot of trouble.
Cambridge protocols
Storage buffer
transformation of R5(2)-mCh-FL-BST and
expression
lysis and immobilization
Bio320 Microbe Species Wikipedia Pages
Getting started with MediaWiki
Consult the User's Guide for information on using the wiki software.