Blue Tongue: Difference between revisions
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PreDec2022>MadeleineGallic No edit summary |
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*Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assay were created to detect eastern or western topotype of BTV. They created primers that recognized 6-distinct sequences of BTV genome segment 1. These assays were able to detect BTV RNA in all positive isolates tested. The sensitivity of RT-LAMP is similar to the sensitivity of RT-PCR but higher than conventional RT-PCR. Could not detect other local viruses that cause symptoms similar to BTV. | *Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assay were created to detect eastern or western topotype of BTV. They created primers that recognized 6-distinct sequences of BTV genome segment 1. These assays were able to detect BTV RNA in all positive isolates tested. The sensitivity of RT-LAMP is similar to the sensitivity of RT-PCR but higher than conventional RT-PCR. Could not detect other local viruses that cause symptoms similar to BTV. | ||
Jung-Yong Yeh, Ji-Hye Lee, Hyun-Ji Seo, Jee-Yong Park, Jin-San Moon, In-Soo Cho, In-Soo Choi,Seung-Yong Park, Chang-Seon Song, Joong-Bok Lee (2011). [https://jcm.asm.org/content/jcm/49/4/1389.full.pdf] | Jung-Yong Yeh, Ji-Hye Lee, Hyun-Ji Seo, Jee-Yong Park, Jin-San Moon, In-Soo Cho, In-Soo Choi,Seung-Yong Park, Chang-Seon Song, Joong-Bok Lee (2011). [[https://jcm.asm.org/content/jcm/49/4/1389.full.pdf Simultaneous Detection of Rift Valley Fever, Bluetongue, Rinderpest, and Peste des Petits Ruminants Viruses by a Single-Tube Multiplex Reverse Transcriptase-PCR Assay Using a Dual-Priming Oligonucleotide System]] | ||
Revision as of 22:06, 1 April 2019
Maan S, Maan NS, Batra K, Kumar A, Gupta A, Rao PP, Hemadri D, Reddy YN, Guimera M, Belaganahalli MN, Mertens PP. “Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India” (ask for access to this paper)
- Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assay were created to detect eastern or western topotype of BTV. They created primers that recognized 6-distinct sequences of BTV genome segment 1. These assays were able to detect BTV RNA in all positive isolates tested. The sensitivity of RT-LAMP is similar to the sensitivity of RT-PCR but higher than conventional RT-PCR. Could not detect other local viruses that cause symptoms similar to BTV.
Jung-Yong Yeh, Ji-Hye Lee, Hyun-Ji Seo, Jee-Yong Park, Jin-San Moon, In-Soo Cho, In-Soo Choi,Seung-Yong Park, Chang-Seon Song, Joong-Bok Lee (2011). [Simultaneous Detection of Rift Valley Fever, Bluetongue, Rinderpest, and Peste des Petits Ruminants Viruses by a Single-Tube Multiplex Reverse Transcriptase-PCR Assay Using a Dual-Priming Oligonucleotide System]