Blue Tongue: Difference between revisions
PreDec2022>MadeleineGallic No edit summary |
PreDec2022>MadeleineGallic No edit summary |
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Maan S, Maan NS, Batra K, Kumar A, Gupta A, Rao PP, Hemadri D, Reddy YN, Guimera M, Belaganahalli MN, Mertens PP. “Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India” (ask for access to this paper) | Maan S, Maan NS, Batra K, Kumar A, Gupta A, Rao PP, Hemadri D, Reddy YN, Guimera M, Belaganahalli MN, Mertens PP. “Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India” (ask for access to this paper) | ||
• Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assay were created to detect eastern or western topotype of BTV. They created primers that recognized 6-distinct sequences of BTV genome segment 1. These assays were able to detect BTV RNA in all positive isolates tested. The sensitivity of RT-LAMP is similar to the sensitivity of RT-PCR but higher than conventional RT-PCR. Could not detect other local viruses that cause symptoms similar to BTV. | |||
Revision as of 22:03, 1 April 2019
Maan S, Maan NS, Batra K, Kumar A, Gupta A, Rao PP, Hemadri D, Reddy YN, Guimera M, Belaganahalli MN, Mertens PP. “Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India” (ask for access to this paper)
• Reverse-transcription-loop-mediated-isothermal-amplification (RT-LAMP) assay were created to detect eastern or western topotype of BTV. They created primers that recognized 6-distinct sequences of BTV genome segment 1. These assays were able to detect BTV RNA in all positive isolates tested. The sensitivity of RT-LAMP is similar to the sensitivity of RT-PCR but higher than conventional RT-PCR. Could not detect other local viruses that cause symptoms similar to BTV.