Streptococcus mutans Growth: Difference between revisions
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= SMSDM = | = SMSDM = | ||
{| class="wikitable" style="margin:auto" | |||
! !! For 500ml (100% vitamins) !! For 500ml (50% vitamins) !! For 3ml (50% vitamins) | |||
|- | |||
| Base || 447.5ml || 449ml || 2.7ml | |||
|- | |||
| Solution I — C+Y vitamins || 3ml || 1.5ml || 9ul | |||
|- | |||
| Solution IB || 2ml || 2ml || 12ul | |||
|- | |||
| Solution II — Metals || 5ml || 5ml || 15ul | |||
|- | |||
| Solution III — Unstables || 2.5ml || 2.5ml || 15ul | |||
|- | |||
| 10% Glucose || 40ml || 40ml || 240ul | |||
|} | |||
== Base Medium == | == Base Medium == | ||
Revision as of 15:14, 18 October 2023
Generic Tips
- Strains can grow on Tryptic Soy Broth plates (TSB) or TSB + Blood plates. There is no hemolysis on blood plates.
- Strains take 2 days to grow on plates from the freezer; please plan accordingly!
- Shaking is not needed. Notice that these cells have a more granular appearance, as they form tight clusters of cells.
- Overnight cultures in BHI begin to die off; please do not plan on using them for more than the next day, if at all.
- Less death is evident in SMSDM (S. mutans Semi-defined Media); same for BHI plates; we notice few cells surviving after 48 hours.
SMSDM
For 500ml (100% vitamins) | For 500ml (50% vitamins) | For 3ml (50% vitamins) | |
---|---|---|---|
Base | 447.5ml | 449ml | 2.7ml |
Solution I — C+Y vitamins | 3ml | 1.5ml | 9ul |
Solution IB | 2ml | 2ml | 12ul |
Solution II — Metals | 5ml | 5ml | 15ul |
Solution III — Unstables | 2.5ml | 2.5ml | 15ul |
10% Glucose | 40ml | 40ml | 240ul |
Base Medium
Base Media 1.11x concentrationCompound | Amount | Final Concentration |
---|---|---|
K2HPO4 | 5.61g | 58mM |
KH2PO4 | 1.13g | 15mM |
(NH4)2SO4 | 734mg | 10mM |
NaCl | 1.14g | 35mM |
Casamino Acids | 1.10g | 0.2% |
- Fill to 450ml
- pH to 7.4