Zone of Inhibition Assay: Difference between revisions
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PreDec2022>Carolin Schmidt No edit summary |
PreDec2022>Carolin Schmidt No edit summary |
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# Add 8 empty 13mm tubes with caps to the block | # Add 8 empty 13mm tubes with caps to the block | ||
# Warm up the BHI agar in the science microwave | # Warm up the BHI agar in the science microwave | ||
a. Start at power level 6 | |||
b. Watch --> if starts to boil lower the power level | |||
c. Should take about 10 min | |||
# When all melted lave to cool for a little so it is not boiling | # When all melted lave to cool for a little so it is not boiling | ||
a. To cool a little faster put in a cool water bath | |||
# Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles) | # Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles) | ||
Note: multiple plate can be made in advance, but don’t leave for longer then a week | Note: multiple plate can be made in advance, but don’t leave for longer then a week | ||
# Label plate | # Label plate |
Revision as of 10:46, 29 October 2019
The night/ day before create liquid cultures of the two bacteria that wish to work with
In the hood:
- Heat bock set to 45 degrees
- Vortexer
- 10ul, 200ul, and 1000ul pipettor
- Auto pipettor
- 10ul, 200ul and 100ul pipet tips
- 24 well plate
Create a beaker of verkon
- Fill a beaker with 500ml of water
- Add two ‘john dear’ spoon full of Verkon
Zone of Inhibition
- Set the heat block to 45 degrees
- Add 8 empty 13mm tubes with caps to the block
- Warm up the BHI agar in the science microwave
a. Start at power level 6
b. Watch --> if starts to boil lower the power level c. Should take about 10 min
- When all melted lave to cool for a little so it is not boiling
a. To cool a little faster put in a cool water bath
- Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles)
Note: multiple plate can be made in advance, but don’t leave for longer then a week
- Label plate