Zone of Inhibition Assay: Difference between revisions

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PreDec2022>Carolin Schmidt
(Created page with "=== The night/ day before  create liquid cultures of the two bacteria that wish to work with === === In the hood: === # Heat bock set to 45 degrees # Vortexer # 10ul, 2...")
 
PreDec2022>Carolin Schmidt
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# Fill a beaker with 500ml of water  
# Fill a beaker with 500ml of water  
# Add two ‘john dear’ spoon full of Verkon
# Add two ‘john dear’ spoon full of Verkon
Zone of Inhibition
=== Zone of Inhibition ===
1. Set the heat block to 45 degrees  
# Set the heat block to 45 degrees  
2. Add 8 empty 13mm tubes with caps to the block
# Add 8 empty 13mm tubes with caps to the block
3. Warm up the BHI agar in the science microwave  
# Warm up the BHI agar in the science microwave  
a. Start at power level 6  
        # Start at power level 6  
b. Watch if starts to boil lower the power level  
# Watch --> if starts to boil lower the power level  
c. Should take about 10 min  
# Should take about 10 min  
4. When all melted lave to cool for a little so it is not boiling  
# When all melted lave to cool for a little so it is not boiling  
a. To cool a little faster put in a cool water bath  
# To cool a little faster put in a cool water bath  
5. Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles)
# Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles)
Note: multiple plate can be made in advance, but don’t leave for longer then a week  
Note: multiple plate can be made in advance, but don’t leave for longer then a week  
6. Label plate
# Label plate

Revision as of 10:45, 29 October 2019

The night/ day before  create liquid cultures of the two bacteria that wish to work with

In the hood:

  1. Heat bock set to 45 degrees
  2. Vortexer
  3. 10ul, 200ul, and 1000ul pipettor
  4. Auto pipettor
  5. 10ul, 200ul and 100ul pipet tips
  6. 24 well plate

Create a beaker of verkon

  1. Fill a beaker with 500ml of water
  2. Add two ‘john dear’ spoon full of Verkon

Zone of Inhibition

  1. Set the heat block to 45 degrees
  2. Add 8 empty 13mm tubes with caps to the block
  3. Warm up the BHI agar in the science microwave
       # Start at power level 6

# Watch --> if starts to boil lower the power level # Should take about 10 min

  1. When all melted lave to cool for a little so it is not boiling

# To cool a little faster put in a cool water bath

  1. Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles)

Note: multiple plate can be made in advance, but don’t leave for longer then a week

  1. Label plate
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