Remote Molecular Biology: Difference between revisions
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qPCR machines: | qPCR machines: | ||
* $300 PCR machine from Dr Brian Kim's group in South Flordia: Unclear if possible to change PCR temperatures. Requires a large amount of reagents (20ul) and 20ul mineral oil instead of a heated lid. Only 1 sample at a time. Matlab might be required to initialize results. | |||
*open PCR machine was bought by Chai; it is no longer commercially made [https://openpcr.org/]. This website does have the maker instructions for it. | *open PCR machine was bought by Chai; it is no longer commercially made [https://openpcr.org/]. This website does have the maker instructions for it. | ||
*Chai makes an open qPCR machine [https://www.chaibio.com/openqpcr]. $4,300 for one channel, $6,000 for dual channel. [https://github.com/chaibio/chaipcr open source code] [https://grabcad.com/library/chai-open-qpcr-1 open source construction] | *Chai makes an open qPCR machine [https://www.chaibio.com/openqpcr]. $4,300 for one channel, $6,000 for dual channel. [https://github.com/chaibio/chaipcr open source code] [https://grabcad.com/library/chai-open-qpcr-1 open source construction] | ||
Revision as of 20:33, 3 March 2019
Topics to Investigate
Articles / Papers to read
Relevant Pathogens
General Lab Methods
PCR-based Methods
Loop-mediated isothermal amplification (LAMP):
- Minireview: Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects
- Simultaneous DNA amplification and detection using a pH-sensing semiconductor system
- LAMP for Human African Trypanosomiasis: A Comparative Study of Detection Formats
- Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
qPCR machines:
- $300 PCR machine from Dr Brian Kim's group in South Flordia: Unclear if possible to change PCR temperatures. Requires a large amount of reagents (20ul) and 20ul mineral oil instead of a heated lid. Only 1 sample at a time. Matlab might be required to initialize results.
- open PCR machine was bought by Chai; it is no longer commercially made [1]. This website does have the maker instructions for it.
- Chai makes an open qPCR machine [2]. $4,300 for one channel, $6,000 for dual channel. open source code open source construction
- Biomeme has a pre-shipment travel qPCR machine with 3 channels. $9,950. [3]