Reagent Recipes: Difference between revisions
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PreDec2022>EricMiller No edit summary |
PreDec2022>Yhahn |
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Line 14: | Line 14: | ||
| Bromo-chloro-indolyl-galactopyranoside | | Bromo-chloro-indolyl-galactopyranoside | ||
| X-gal | | X-gal | ||
| | | 20mg/ml | ||
| Dimethylformamide (DMF) | | Dimethylformamide (DMF) | ||
| | | 250x | ||
| Use DMF in a chemical hood | | Use DMF in a chemical hood | ||
|- | |- | ||
Line 26: | Line 26: | ||
| Light sensitive. Molecular weight: 238.31 g/mol | | Light sensitive. Molecular weight: 238.31 g/mol | ||
|} | |} | ||
=Glycerol for freezing aliquots= | =Glycerol for freezing aliquots= |
Revision as of 10:27, 25 February 2019
Reagent Information
Reagent | Abbreviation | Stock Solution
Concentration |
Solvent | Stock Concentration to
Working Concentration |
Notes |
---|---|---|---|---|---|
Bromo-chloro-indolyl-galactopyranoside | X-gal | 20mg/ml | Dimethylformamide (DMF) | 250x | Use DMF in a chemical hood |
Isopropyl β-D-1-thiogalactopyranoside | IPTG | 0.1M | H2O | 1000x | Light sensitive. Molecular weight: 238.31 g/mol |
Glycerol for freezing aliquots
We use sterile 80% glycerol in water. We dilute this 1:3 (400ul 80% glycerol; 1200ul culture) for creating freezer stocks with a final glycerol concentration of 20%.
10x PBS
Used to create 1x PBS for dilution or washing cells of any species.
For 1 liter:
- NaCl 80g
- KCl 2g
- Na2HPO4 14.4g
- KH2PO4 2.4g
Stir salts with MilliQ water. This may take a significant time to dissolve!
Adjust pH with NaOH to 7.4. This will take a significant amount of NaOH; add in 200ul increments.
Tris Buffer
For Tris buffer at pH 8.0 (at 25 degrees)
- 4.44 g/L Tris HCl
- 2.65 g/L Tris Base
Do not adjust pH or check the pH with the MEE lab pH probe. This will destroy the probe!