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== General microbiology protocols == | == General microbiology protocols == | ||
===[[Media Recipes]]=== | ===[[Media Recipes]]=== | ||
| Line 10: | Line 5: | ||
===[[Freezing -80 Stocks]]=== | ===[[Freezing -80 Stocks]]=== | ||
===[[Freezing Aliquots]]=== | ===[[Freezing Aliquots]]=== | ||
===[[Competition Assays]]=== | ===[[Competition Assays]]=== | ||
===[[Generic PCR]]=== | ===[[Generic PCR]]=== | ||
===[[Running DNA Gels]]=== | ===[[Running DNA Gels]]=== | ||
== Cloning and gene manipulation == | |||
===[[Commonly Used Plasmids]=== | |||
===[[Plasmid Purification]]=== | |||
===[[Digest and Ligation]]=== | ===[[Digest and Ligation]]=== | ||
===[[Creating Competent E. coli Cells]]=== | |||
===[[Transformation]]=== | ===[[Transformation]]=== | ||
Revision as of 22:19, 12 June 2019
General microbiology protocols
Media Recipes
Reagent Recipes
Working with Antibiotics
Freezing -80 Stocks
Freezing Aliquots
Competition Assays
Generic PCR
Running DNA Gels
Cloning and gene manipulation
[[Commonly Used Plasmids]
Plasmid Purification
Digest and Ligation
Creating Competent E. coli Cells
Transformation
Phyllosphere protocols
Creating Sterile Agar Plates
Sterilization and Germination Protocol for ''Arabidopsis thaliana'' Seeds in Gnotobiotic Experiments
Germination Protocol for ''Arabidopsis thaliana'' Seeds in Non-Sterile Experiments
Growth Stage Phenotype Definitions
Growth Conditions for ''Arabidopsis thaliana''
Measuring Light with HOBO Data Loggers
Inoculation of ''Arabidopsis thaliana'' with Microbes
Removal and DNA Extraction of Phyllosphere Microbes
ARISA
Measuring ''A. thaliana'' Phenotype using FIJI
Streptococcus pneumoniae protocols
Dual Layer Assays
Streptococcus DNA Extraction
Streptococcus suis protocols
Measuring Absorbance in Streptococcus
Streptococcus DNA Extraction
Streptococcus Competence Induction
Peptide Synthesis
Peptide Cleavage
Mass Spectrometery
Plate Reader Assay and Growth Curve
Measuring Competence : Fixation and Flow Cytometry
Remote Molecular Biology
Effect of Laboratory Protocols on Student Learning
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