Latest revision as of 14:41, 15 May 2024

Generic Tips

Strains can grow on Tryptic Soy Broth plates (TSB) or TSB + Blood plates. There is no hemolysis on blood plates.
Strains are usually grown on Brain Heart Infusion (BHI).
Strain s-763 take 2 days to grow on plates from the freezer; please plan accordingly! Other strains take ~24 hours to grow.
SMUR is a low peptide liquid media used for transformation, BHI is used otherwise

Shaking is not needed. Notice that these cells have a more granular appearance, as they form tight clusters of cells.
Overnight cultures in BHI begin to die off; please do not plan on using them for more than the next day, if at all.
Less death is evident in SMSDM (S. mutans Semi-defined Media); same for BHI plates; we notice few cells surviving after 48 hours.

Base Media 1.11x concentration

@@ Line 1: / Line 1: @@
 = Generic Tips =
 * Strains can grow on Tryptic Soy Broth plates (TSB) or TSB + Blood plates. There is no hemolysis on blood plates.
-* Strains take 2 days to grow on plates from the freezer; please plan accordingly!
+* Strains are usually grown on Brain Heart Infusion (BHI).
+* Strain s-763 take 2 days to grow on plates from the freezer; please plan accordingly! Other strains take ~24 hours to grow.
+* SMUR is a low peptide liquid media used for transformation, BHI is used otherwise
 * Shaking is not needed. Notice that these cells have a more granular appearance, as they form tight clusters of cells.
@@ Line 8: / Line 10: @@
 = SMSDM =
+{| class="wikitable" style="margin:auto"
+!  !! For 500ml (100% vitamins) !! For 500ml (50% vitamins) !! For 3ml (50% vitamins)
+|-
+| Base || 447.5ml || 449ml || 2.7ml
+|-
+| Solution I — C+Y vitamins || 3ml || 1.5ml || 9ul
+|-
+| Solution IB || 2ml || 2ml || 12ul
+|-
+| Solution II — Metals || 5ml || 5ml || 15ul
+|-
+| Solution III — Unstables || 2.5ml || 2.5ml || 15ul
+|-
+| 10% Glucose || 40ml || 40ml || 240ul
+|}
 == Base Medium ==