Remote Molecular Biology: Difference between revisions

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==[[Potential Grants]]==
==[[Topics to Investigate]]==
==[[Topics to Investigate]]==


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==[[PCR-based Methods]]==
==[[PCR-based Methods]]==


==[[Fluorescent Tagging of Primers]]==


==[[Magnetic Beads for DNA isolation]]==


Loop-mediated isothermal amplification (LAMP):
==[[Freezing PCR reagents]]==
 
*[https://link.springer.com/content/pdf/10.1007%2Fs12275-015-4656-9.pdf Minireview: Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects]
*[https://www.nature.com/articles/nmeth.2520.pdf Simultaneous DNA amplification and detection using a pH-sensing semiconductor system]
*[https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000865&type=printable LAMP for Human African Trypanosomiasis: A Comparative Study of Detection Formats]
*[https://www.future-science.com/doi/pdf/10.2144/000114253 Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes]
 
 


qPCR machines:
==[[Producing Nucleotides for PCR]]==
*open PCR machine was bought by Chai; it is no longer commercially made [https://openpcr.org/]. This website does have the maker instructions for it.
*Chai makes an open qPCR machine [https://www.chaibio.com/openqpcr]. $4,300 for one channel, $6,000 for dual channel. [https://github.com/chaibio/chaipcr open source code] [https://grabcad.com/library/chai-open-qpcr-1 open source construction]
* Biomeme has a pre-shipment travel qPCR machine with 3 channels. $9,950. [https://shop.biomeme.com/collections/devices/products/three9-real-time-pcr-thermocycler-pre-order?variant=656540172300]


==[[Culture-based Methods]]==
==[[Culture-based Methods]]==

Latest revision as of 12:57, 16 December 2022