Streptococcus mutans Transformation: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 10: | Line 10: | ||
Day 3: Transformations | Day 3: Transformations | ||
#Add 1500uL of SMUR to a well of the 96-well plate | # Add 1500uL of SMUR to a well of the 96-well plate | ||
## Have one well set aside for | ## Have one well set aside for a negative control, no XIP or DNA to this well | ||
# Pipette up and down the overnight culture with 500uL. Immediately after add 15uL of the overnight into SMUR wells. | |||
Day 4: Plating Transformations | Day 4: Plating Transformations | ||
Revision as of 14:31, 15 May 2024
Day 1: Streaking Out Plates
- Warm 1 BHI Plate
- Triple streak out S. mutans from freezer
- Allow overnight growth in a 37˚C 5% CO2 incubator
Day 2: Overnight Growth
- Add 1500uL of BHI to a well of the 96-well plate
- Add ~5 colonies of S. mutans to the BHI
- Allow overnight growth in a 37˚C 5% CO2 incubator
Day 3: Transformations
- Add 1500uL of SMUR to a well of the 96-well plate
- Have one well set aside for a negative control, no XIP or DNA to this well
- Pipette up and down the overnight culture with 500uL. Immediately after add 15uL of the overnight into SMUR wells.
Day 4: Plating Transformations