Streptococcus mutans Transformation: Difference between revisions
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(Replaced content with "Day 1: Streaking Out Plates # Warm 1 BHI Plate # Triple streak out S. mutans from freezer Day 2: Overnight Growth Day 3: Transformations Day 4: Plating Transformations") Tag: Replaced |
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# Warm 1 BHI Plate | # Warm 1 BHI Plate | ||
# Triple streak out S. mutans from freezer | # Triple streak out S. mutans from freezer | ||
# Allow overnight growth in a 37˚C 5% CO2 incubator | |||
Day 2: Overnight Growth | Day 2: Overnight Growth | ||
# Add 1500uL of BHI to a well of the 96-well plate | |||
# Add ~5 colonies of S. mutans to the BHI | |||
# Allow overnight growth in a 37˚C 5% CO2 incubator | |||
Day 3: Transformations | Day 3: Transformations | ||
#Add 1500uL of SMUR to a well of the 96-well plate | |||
## Have one well set aside for | |||
Day 4: Plating Transformations | Day 4: Plating Transformations | ||
Revision as of 14:29, 15 May 2024
Day 1: Streaking Out Plates
- Warm 1 BHI Plate
- Triple streak out S. mutans from freezer
- Allow overnight growth in a 37˚C 5% CO2 incubator
Day 2: Overnight Growth
- Add 1500uL of BHI to a well of the 96-well plate
- Add ~5 colonies of S. mutans to the BHI
- Allow overnight growth in a 37˚C 5% CO2 incubator
Day 3: Transformations
- Add 1500uL of SMUR to a well of the 96-well plate
- Have one well set aside for
Day 4: Plating Transformations