Streptococcus mutans Transformation Media Recipe (SMUR): Difference between revisions
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45mL 100x Metals Solution: | 45mL 100x Metals Solution: | ||
[[File:Metals_SMUR.png]] | [[File:Metals_SMUR.png]] | ||
Add MgCl2 last and make sure everything is dissolved before it is added. There will be an exothermic | |||
reaction and and color change upon addition of MgCl2. If there is trouble dissolving everything, | |||
heat water bath to 45˚C and heat solution while vortexing occasionally. Then filer sterilize. | |||
40mL 500x Vitamins Solution: | 40mL 500x Vitamins Solution: | ||
[[File:V_SMUR.png]] | [[File:V_SMUR.png]] | ||
If there is trouble dissolving, heat water bath to 45˚C and heat solution while vortexing | |||
occasionally. Then filer sterilize. | |||
40mL 100x Unstables Solution: | 40mL 100x Unstables Solution: | ||
[[File:U_SMUR.png]] | [[File:U_SMUR.png]] | ||
If there is trouble dissolving, heat water bath to 37˚C and heat solution while vortexing | |||
occasionally. Then filer sterilize. | |||
Revision as of 13:58, 15 May 2024
S. mutans Recombination (SMUR) Media Recipe:
Final Recipe:
Stock Solutions:
500mL 1.5x Base Media:
45mL 100x Metals Solution:
Add MgCl2 last and make sure everything is dissolved before it is added. There will be an exothermic
reaction and and color change upon addition of MgCl2. If there is trouble dissolving everything,
heat water bath to 45˚C and heat solution while vortexing occasionally. Then filer sterilize.
40mL 500x Vitamins Solution:
If there is trouble dissolving, heat water bath to 45˚C and heat solution while vortexing
occasionally. Then filer sterilize.
40mL 100x Unstables Solution:
If there is trouble dissolving, heat water bath to 37˚C and heat solution while vortexing
occasionally. Then filer sterilize.