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This Week in Microbiology
- By Vincent Racaniello
- 5 stars! Amazing podcast to learn all about different types of microbiology research! This podcast goes pretty in depth into different current scientific papers so it is a great way to learn about current research.

This Week in Virology
- By Vincent Racaniello

Ologies
- By Alie Ward
- 5 stars! Great all around podcast made for a more general audience. There are many different episodes on scientific topics including Environmental Microbiology, Mathematical Biology, and much much more. This is a great podcast to explore different careers and listen to some amazing speakers.

Overheard at National Geographic
- By National Geographic
- 4 stars! Great podcast to hear about the many wonders of the world. It also has shorter episodes and is a great way to learn about the world.

Journey to the Micro Cosmos
- 3 stars! Short 10 minute episodes about cool microbes.

Waste Disposal

Streptococcus suis protocols

Streptococcus suis Transformation

Measuring Absorbance in Streptococcus

Streptococcus DNA Extraction

Streptococcus Competence Induction

Peptide Synthesis

Peptide Cleavage

Mass Spectrometery

Plate Reader Assay and Growth Curve

Measuring Competence : Fixation and Flow Cytometry

Arabidopsis thaliana protocols

Creating Sterile Agar Plates

Sterile Seeding Protocol

Germination Protocol for ''Arabidopsis thaliana'' Seeds in Non-Sterile Experiments

@@ Line 85: / Line 85: @@
 ===[[Media and Passaging]]===
 ===[[Plaque Assays with Soft Agar]]===
-'''The soft agar should be prepared first, likely at least an hour before you pour'''
-# Put a heating block in the hood; set to 42C and place sterile 13mm test tubes in it to warm up; use the number of plates expected, plus 1 test tube.
-# Make sure the water bath has enough water and is warming to 42C
-#* You will use about 4 mL of soft agar per plate, so calculate total volume of agar needed accordingly
-#* Always prepare enough agar for at least one extra plate
-# Loosen the cap of the soft agar bottle and microwave the bottle of agar until it is fully liquid:
-#* Check for bubbling about every 15 seconds
-#* You will know when it is fully melted because:
-#** There will be no more “cloudiness” or bits
-#** Shaking or swirling will cause bubbles to rise directly to the top(not being blocked by solid material)
-# After microwaving, place bottle into the preheated water bath
-# After preparing agar, use a serological pipet to transfer 4 mL of soft agar into each 13mm test tube. The top layer on plates will consist of an equally sized layer made of the same broth and soft agar (0.8% agar) mixed with 60 uL of bacterial culture.
-# Warm to 37 degrees and label plates with circles and dilution numbers to make plating easier
-#* Phage dilution will depend on goals of the assay but normally it consists of the undiluted sample and a range from 10^-2 to 10^-4
-# Prepare for pouring the phage layer
-#* Prepare all phage pipettes
-#* Prepare filtered tips for phage
-#* Check that your dilutions are represent and organized
-#* Check that you collected all your warmed plates from the incubator
-#* Make sure your vortexer is in the hood, plugged in and the outlet is switched on
-# Quickly but carefully (1 by 1):
-#* Pipette 60 uL of overnight bacterial culture into 13mm test tube
-#* Vortex for about 5 seconds (look for the tornado)
-#* Open pre-labeled plate and pour mixture onto the LB layer
-#* Swirl gently to allow soft agar to spread evenly, cover, then set aside
-#* Allow about 15 seconds for a poured plate to set before moving
-# Plate 5 μl of each phage sample and dilution according to previously made circles
-#* After adding phage we need to allow liquid to dry since it is on top of the solidified soft agar. While it would dry faster if the plate was uncovered, we do not want the phage to contaminate the hood in any way, so let liquid dry with the lid on the plate.
-# Allow to dry for about 1 hour to ensure no drying issues
-# Place plates in 37C incubator and check the next day.
 ===[[Serial Dilutions of Phage]]===
 ===[[Calculating Virus Titre]]===

Revision as of 12:36, 8 January 2024

General microbiology protocols

Cloning and gene manipulation

Streptococcus pneumoniae protocols

Streptococcus mutans protocols

Myxococcus xanthus protocols

Phage protocols

Interesting Podcasts to Listen to When Doing Lab Work!

Streptococcus suis protocols

Arabidopsis thaliana protocols

Cambridge protocols

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