Serial Dilutions of Phage: Difference between revisions
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1. Label 8, microcentrifuge tubes with 10^ | 1. Label 8, microcentrifuge tubes with 10^1, 10^2,10^3 all the way down to 10^8 (These will serve as our dilution tubes) | ||
2. Add 900 uL of LB broth to each tube | 2. Add 900 uL of LB broth to each tube | ||
| Line 5: | Line 5: | ||
3. Vortex the phage stock. | 3. Vortex the phage stock. | ||
4. To the first tube (10^ | 4. To the first tube (10^1), add 100 uL of phage stock and vortex | ||
5. Change the pippette tip. | 5. Change the pippette tip. | ||
6. To the second tube (10^ | 6. To the second tube (10^2), take 100 uL of tube 1, add it to the next dilution, and vortex the second tube | ||
7. Repeat step 5 and 6 down to the 10^-8 tube | 7. Repeat step 5 and 6 down to the 10^-8 tube | ||
8 Double check your work by making sure tubes 1-7 have 900 uL of liquid in them, and the last should have 1 mL | 8 Double check your work by making sure tubes 1-7 have 900 uL of liquid in them, and the last should have 1 mL | ||
Latest revision as of 13:38, 19 March 2023
1. Label 8, microcentrifuge tubes with 10^1, 10^2,10^3 all the way down to 10^8 (These will serve as our dilution tubes)
2. Add 900 uL of LB broth to each tube
3. Vortex the phage stock.
4. To the first tube (10^1), add 100 uL of phage stock and vortex
5. Change the pippette tip.
6. To the second tube (10^2), take 100 uL of tube 1, add it to the next dilution, and vortex the second tube
7. Repeat step 5 and 6 down to the 10^-8 tube
8 Double check your work by making sure tubes 1-7 have 900 uL of liquid in them, and the last should have 1 mL