Zone of Inhibition Assay

In the hood:

1. Heat bock set to 45 degrees
2. Vortexer
3. 10ul, 200ul, and 1000ul pipettor
4. Auto pipettor
5. 10ul, 200ul and 100ul pipet tips
6. 24 well plate

Create a beaker of verkon

1. Fill a beaker with 500ml of water
2. Add two ‘john dear’ spoon full of Verkon

Zone of Inhibition

1. Set the heat block to 45 degrees
2. Add 8 empty 13mm tubes with caps to the block
3. Warm up the BHI agar in the science microwave

       a. Start at power level 6 

b. Watch --> if starts to boil lower the power level c. Should take about 10 min

1. When all melted lave to cool for a little so it is not boiling

a. To cool a little faster put in a cool water bath

1. Add 1ml of agar to each well of a 24 well plate (carefully to not form bubbles)

Note: multiple plate can be made in advance, but don’t leave for longer then a week

1. Label plate