Working with Antibiotics: Difference between revisions

Revision as of 13:28, 19 October 2018

Antibiotic Information

Antibiotic	Appreviation	Stock Solution Concentration	Solvent	Stock Concentration to Working Concentration	Notes
Ampicillin	Amp	100mg/ml	H2O	1000x
Carbenicillin	Crb	100mg/ml	50% Ethanol	1000x
Chloramphenicol	Cam	20mg/ml	100% Ethanol	1000x
Cycloheximide	Chx	100mg/ml	DMSO	1000x	Toxic to fungi and animals (including humans) Use protective gear; work in chemical hood.
Erythromycin	Ery	250mg/ml	100% Ethanol	1000x
Kanamycin	Kan	50mg/ml	H2O	1000x
Spectinomycin	Spec	60mg/ml	H2O	1000x
Streptomycin	Str	100mg/ml	H2O	1000x
Tetracycline	Tet	10mg/ml	70% Ethanol	1000x	Light sensitive Wrap tubes and plates in aluminum foil

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-Stock solution concentrations:
+Antibiotic Information
-*Penicillin 100 mg/mL, solvent MQ water
-*Tetracycline 50 mg/mL, solvent MQ water
-*Erythromycin 50 mg/mL, solvent Ethanol
-Final concentrations for plates:
-Final concentrations for broth:
-Col-0 Data
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