Silica Microparticle Biosensor: Difference between revisions
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PreDec2022>TrevorE (Created page with "Gene to Diagnostic: Self immobilizing protein for silica microparticle biosensor, modelled with sarcosine oxidase. -Monitoring Protein Expression with an increase in mCherry...") |
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Gene to Diagnostic: Self immobilizing protein for silica microparticle biosensor, | Gene to Diagnostic: Self-immobilizing protein for silica microparticle biosensor, modeled with sarcosine oxidase. | ||
-Monitoring Protein Expression with an increase in mCherry fluorescence | -Monitoring Protein Expression with an increase in mCherry fluorescence | ||
-R5 Peptide | -R5 Peptide | ||
-immobilized protein stable for more than 2 months at RTP | -immobilized protein stable for more than 2 months at RTP | ||
-silica acts | -silica acts immobilization encapsulant while retaining the activity of biomolecules | ||
Main Components | Main Components | ||
1) the central functional assay protein | 1) the central functional assay protein (enzyme e.g Monomeric Sarcosine Oxidase/mSox) | ||
2) a visualizing protein ( fluorescent protein) | 2) a visualizing protein ( fluorescent protein) | ||
-they used mCherry | -they used mCherry | ||
Revision as of 14:32, 26 March 2019
Gene to Diagnostic: Self-immobilizing protein for silica microparticle biosensor, modeled with sarcosine oxidase.
-Monitoring Protein Expression with an increase in mCherry fluorescence -R5 Peptide -immobilized protein stable for more than 2 months at RTP -silica acts immobilization encapsulant while retaining the activity of biomolecules
Main Components
1) the central functional assay protein (enzyme e.g Monomeric Sarcosine Oxidase/mSox)
2) a visualizing protein ( fluorescent protein)
-they used mCherry -GFP showed no significant absorption to silica nanoparticles unless denatured - Red fluorescent protein slightly better than GFP but must also be denatured
3)In-built immobilization peptide
4) possible platforms: sillica, paper, metals, carbon
- dependant on type of peptide chosen
Process
-They designed the visualizing proteins
-Silica isolation (less than 5 g of sand needed)
requires centrifugation takes 5 days
-immobilization of Protein
sonicated (1 hour) rotated (1 hour) calculated % of immobilization through Bradford Assay and fluorescence intensity
HourGlass Biosensor Method