Peste Des Petits Ruminants: Difference between revisions
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*this assay was based on rapid purification of RNA on glass beads followed by RT-PCR. They used a set of primers created to target NP3/NP4 that encodes for the nucleocapsidase protein. The PCR was then detected using UV illumination after agarose gel electrophoresis or by hybridization using a oligonucleotide probe after blot transfer. They found that this technique was 1000-fold more sensitive than convention titration techniques. | *this assay was based on rapid purification of RNA on glass beads followed by RT-PCR. They used a set of primers created to target NP3/NP4 that encodes for the nucleocapsidase protein. The PCR was then detected using UV illumination after agarose gel electrophoresis or by hybridization using a oligonucleotide probe after blot transfer. They found that this technique was 1000-fold more sensitive than convention titration techniques. | ||
LinLi, JingyueBao, XiaodongWu, ZhiliangWang, JunweiWang, MingxiaGong, ChunjuLiu, JinmingLi (2010). [https://www.ncbi.nlm.nih.gov/pubmed/20813134 | |||
Rapid detection of peste des petits ruminants virus by a reverse transcription loop-mediated isothermal amplification assay.] ask for access to this | |||
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*This assay seems like it is detecting pestes de petits ruminants virus which is the causative agent of peste de petits. A set of 6 primers were created to amplify the target RNA by one single temperature incubation period. They tested this assay by amplifying 8 strains of PPRV and then the amplified products could be visualized by the naked eye. Additionally, they saw no cross-reactivity with other related viruses. The sensitivity of this assay was equivalent to RT-PCR and 10x higher than conventional RT-PCR. | |||
Revision as of 21:09, 15 April 2019
E Couacy-Hymann, F Roger, C Hurard, J.P Guillou, G Libeau, A Diallo. (2002). Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay need access.
- this assay was based on rapid purification of RNA on glass beads followed by RT-PCR. They used a set of primers created to target NP3/NP4 that encodes for the nucleocapsidase protein. The PCR was then detected using UV illumination after agarose gel electrophoresis or by hybridization using a oligonucleotide probe after blot transfer. They found that this technique was 1000-fold more sensitive than convention titration techniques.
LinLi, JingyueBao, XiaodongWu, ZhiliangWang, JunweiWang, MingxiaGong, ChunjuLiu, JinmingLi (2010). [https://www.ncbi.nlm.nih.gov/pubmed/20813134
Rapid detection of peste des petits ruminants virus by a reverse transcription loop-mediated isothermal amplification assay.] ask for access to this
- This assay seems like it is detecting pestes de petits ruminants virus which is the causative agent of peste de petits. A set of 6 primers were created to amplify the target RNA by one single temperature incubation period. They tested this assay by amplifying 8 strains of PPRV and then the amplified products could be visualized by the naked eye. Additionally, they saw no cross-reactivity with other related viruses. The sensitivity of this assay was equivalent to RT-PCR and 10x higher than conventional RT-PCR.