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===[[Streptococcus DNA Extraction]]=== | ===[[Streptococcus DNA Extraction]]=== | ||
===[[Streptococcus Growth Curve Protocol]]=== | ===[[Streptococcus Growth Curve Protocol]]=== | ||
1. Turn on the Bio safety level 2 hood, and spray down with ethanol | |||
2. Spray gloves with ethanol and wipe inside of hood down | |||
3. Steps 4-6 should be conducted in the hood | |||
4. Set up 6 13mm tubs labeled S. Pneumonia 1-6 and add 3ml of Tryptic soy broth media to each | |||
5. Remove the aliquots from the -20 degree freezer and let them thaw | |||
6. Pipet 30ul of the aliquots into each tube and vortex | |||
7. Leave the tubes in 34 degree, 5% CO2 incubator for 2 hours | |||
8. Remove the first tube, vortex (in the hood) and place in the spectrometer | |||
9. Note: make use to have set up the spectrometer by blanking it | |||
10. Record the absorbance and return the tube to the incubator | |||
11. Later remove the first and second tube from the incubator | |||
12. Vortex (in the hood) and place in the spectrometer | |||
13. Record the absorbance and return tubes to incubator | |||
14. Repeat this with each successive tube | |||
Modified from: Iannelli, F. & Pozzi, G. Mol Biotechnol (2004) 26: 81. https://doi.org/10.1385/MB:26:1:81 | |||
== ''Streptococcus suis'' protocols == | == ''Streptococcus suis'' protocols == |
Revision as of 09:14, 2 October 2019
General microbiology protocols
Media Recipes
Reagent Recipes
Working with Antibiotics
Freezing -80 Stocks
Freezing Aliquots
Competition Assays
Generic PCR
Running DNA Gels
Cloning and gene manipulation
Commonly Used Plasmids
Plasmid Purification
Digest and Ligation
Creating Competent E. coli Cells
Transformation
Gibson Assembly
Phyllosphere protocols
Creating Sterile Agar Plates
Sterilization and Germination Protocol for ''Arabidopsis thaliana'' Seeds in Gnotobiotic Experiments
Germination Protocol for ''Arabidopsis thaliana'' Seeds in Non-Sterile Experiments
Growth Stage Phenotype Definitions
Growth Conditions for ''Arabidopsis thaliana''
Measuring Light with HOBO Data Loggers
Inoculation of ''Arabidopsis thaliana'' with Microbes
Removal and DNA Extraction of Phyllosphere Microbes
ARISA
Measuring ''A. thaliana'' Phenotype using FIJI
Streptococcus pneumoniae protocols
Dual Layer Assays
Streptococcus DNA Extraction
Streptococcus Growth Curve Protocol
1. Turn on the Bio safety level 2 hood, and spray down with ethanol
2. Spray gloves with ethanol and wipe inside of hood down
3. Steps 4-6 should be conducted in the hood
4. Set up 6 13mm tubs labeled S. Pneumonia 1-6 and add 3ml of Tryptic soy broth media to each
5. Remove the aliquots from the -20 degree freezer and let them thaw
6. Pipet 30ul of the aliquots into each tube and vortex
7. Leave the tubes in 34 degree, 5% CO2 incubator for 2 hours
8. Remove the first tube, vortex (in the hood) and place in the spectrometer
9. Note: make use to have set up the spectrometer by blanking it
10. Record the absorbance and return the tube to the incubator
11. Later remove the first and second tube from the incubator
12. Vortex (in the hood) and place in the spectrometer
13. Record the absorbance and return tubes to incubator
14. Repeat this with each successive tube
Modified from: Iannelli, F. & Pozzi, G. Mol Biotechnol (2004) 26: 81. https://doi.org/10.1385/MB:26:1:81
Streptococcus suis protocols
Streptococcus suis Transformation
Measuring Absorbance in Streptococcus
Streptococcus DNA Extraction
Streptococcus Competence Induction
Peptide Synthesis
Peptide Cleavage
Mass Spectrometery
Plate Reader Assay and Growth Curve
Measuring Competence : Fixation and Flow Cytometry
Remote Molecular Biology
Effect of Laboratory Protocols on Student Learning
Cambridge protocols
Storage buffer
transformation of R5(2)-mCh-FL-BST and
expression
lysis and immobilization
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