Infectious Bursal disease: Difference between revisions

Revision as of 21:12, 15 April 2019

The researchers compared RT-LAMP against RT-PCR as detection methods for IBD. Their results showed that RT-LAMP is found to have 10 times higher selectivity for IBDV when compared to RT-LAMP and was able to detect 9.2% more field samples than by RT-PCR. They were able to declare a field sample positive based on the turbidity or by adding fluorescence staining reagent like SYBR green I. they created 6 primers for RT-LAMP which were specific for the VP5 gene (two inner and two outer primers as well as loop primers). They additionally provide the primers they used for RT-PCR. There was no need for a thermos cycler or other heavy instruments with RT-LAMP and a fluorescent dsDNA intercalating dye was used for visual detection.

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 To investigate: [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062922/ One-step reverse-transcription loop-mediated isothermal amplification for detection of infectious bursal disease virus]
+Khan RSA, Ali W, Kiran S, Shah MSD, Tahir ZA, Habib M. (2018). [https://www.ncbi.nlm.nih.gov/pubmed/30046320 Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis.]
+*The researchers compared RT-LAMP against RT-PCR as detection methods for IBD. Their results showed that RT-LAMP is found to have 10 times higher selectivity for IBDV when compared to RT-LAMP and was able to detect 9.2% more field samples than by RT-PCR. They were able to declare a field sample positive based on the turbidity or by adding fluorescence staining reagent like SYBR green I. they created 6 primers for RT-LAMP which were specific for the VP5 gene (two inner and two outer primers as well as loop primers). They additionally provide the primers they used for RT-PCR. There was no need for a thermos cycler or other heavy instruments with RT-LAMP and a fluorescent dsDNA intercalating dye was used for visual detection.