Enzootic Bovine Leukosis: Difference between revisions
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PreDec2022>MadeleineGallic (Created page with "Yuka Okuwa, Michiko Miyamato-Hayashi, Takaichi Tanaka, Yuji Hayakawa and Yasuo Inoshima (2016). [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5289250/pdf/jvms-79-137.pdf Simpl...") |
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*the researchers performed various techniques to see which of the tests was most effective. The researchers performed RT-PCR, nested PCR and LAMP. LAMP assay was compared using DNA extracts from whole blood instead of white blood cells. This is a more simple and rapid way to detect BLV as opposed to using DNA from WBCs. Unfortunately, the researchers did not provide the primers used. IN conclusion they found that LAMP assay using blood DNA was the most suitable for rapid and simple screenings of BLV. | *the researchers performed various techniques to see which of the tests was most effective. The researchers performed RT-PCR, nested PCR and LAMP. LAMP assay was compared using DNA extracts from whole blood instead of white blood cells. This is a more simple and rapid way to detect BLV as opposed to using DNA from WBCs. Unfortunately, the researchers did not provide the primers used. IN conclusion they found that LAMP assay using blood DNA was the most suitable for rapid and simple screenings of BLV. | ||
ChihoKomiyama, KazuhikoSuzuki, YasuoMiura, HiroshiSentsui (2009). [https:// | ChihoKomiyama, KazuhikoSuzuki, YasuoMiura, HiroshiSentsui (2009). [https://drive.google.com/open?id=1lRyix_80ROdob_h7uAm4P2HrL5t2-xOy Development of loop-mediated isothermal amplification method for diagnosis of bovine leukemia virus infection] | ||
*the researchers compared LAMP and PCR as detection methods for BLV. The results showed that LAMP had a higher sensitivity in detecting BLV as compared to PCR. Additionally, the researchers collected the infected cows blood after 6 months, the BLV antibody was detected. This suggested that the LAMP assay could help in detecting BLV in cattle in the early stages of BLV infections. | *the researchers compared LAMP and PCR as detection methods for BLV. The results showed that LAMP had a higher sensitivity in detecting BLV as compared to PCR. Additionally, the researchers collected the infected cows blood after 6 months, the BLV antibody was detected. This suggested that the LAMP assay could help in detecting BLV in cattle in the early stages of BLV infections. | ||
Revision as of 10:28, 23 April 2019
Yuka Okuwa, Michiko Miyamato-Hayashi, Takaichi Tanaka, Yuji Hayakawa and Yasuo Inoshima (2016). Simple and rapid method for routine screening of bovine leukemia virus by loopmediated isothermal amplification assay
- the researchers performed various techniques to see which of the tests was most effective. The researchers performed RT-PCR, nested PCR and LAMP. LAMP assay was compared using DNA extracts from whole blood instead of white blood cells. This is a more simple and rapid way to detect BLV as opposed to using DNA from WBCs. Unfortunately, the researchers did not provide the primers used. IN conclusion they found that LAMP assay using blood DNA was the most suitable for rapid and simple screenings of BLV.
ChihoKomiyama, KazuhikoSuzuki, YasuoMiura, HiroshiSentsui (2009). Development of loop-mediated isothermal amplification method for diagnosis of bovine leukemia virus infection
- the researchers compared LAMP and PCR as detection methods for BLV. The results showed that LAMP had a higher sensitivity in detecting BLV as compared to PCR. Additionally, the researchers collected the infected cows blood after 6 months, the BLV antibody was detected. This suggested that the LAMP assay could help in detecting BLV in cattle in the early stages of BLV infections.