Streptococcus DNA Extraction - Genome Prep: Revision history

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21 June 2024

14 September 2023

  • curprev 13:4913:49, 14 September 2023‎ EricMiller talk contribs‎ 5,639 bytes +5,639‎ Created page with "== Reagents Needed== * 50mM Tris-10mM EDTA, pH 8 Autoclaved. **Note: max concentration that will dissolve EDTA (without altering pH) is 0.1M. Making 10mL of the 50mM Tris Buffer Solution + 10mM of the EDTA will result in a 10% diluted Tris Buffer solution. We are working to troubleshoot this problem for the next time around. **If working with small volumes such as 10mL that need to be sterilized, an alternative to autoclaving is filter sterilization. Use either a 10 or..."
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