Spore Assay: Revision history

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2 May 2024

29 March 2024

25 February 2024

  • curprev 13:5413:54, 25 February 2024‎ Jcomstock talk contribs‎ 3,976 bytes +3,976‎ Created page with "1. Harvest overnight M. xanthus culture during log growth phase, wash twice in TPM, and concentrate to %x10^9 cells/ml. You will likely need several mL of culture to plate out a spore assay. Each large TPM plate will need 4 groups of 5 20uL spots (400uL per plate, where one plate = 1 replicate). You may wish to use the 15mL conical tubes and the centrifuge in superlab to prepare cells. 2. While you are washing and preparing cells, leave TPM plates open to dry by flame o..."
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