Propidium Iodide Staining on Agar Plates: Revision history

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27 October 2023

  • curprev 16:2316:23, 27 October 2023‎ Jcomstock talk contribs‎ 1,018 bytes +1,018‎ Created page with "This protocol is for staining fruiting bodies on agar/agarose plates with propidium iodide (PI). 1. Put on gloves to prevent the PI stain from contacting your skin. 2. Remove an aliquot of PI stain (one of the PCR tubes of purple PI stain) from the -20C freezer. 3. Once thawed, dilute the PI stain 1:100 in TPM (2uL into 198uL for example). You need 20ul of diluted PI solution per spot that you want to stain, so make sure to calculate enough. 4. Mix the PI stain t..."
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