Measuring Burst Size: Revision history

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8 January 2024

  • curprev 12:4212:42, 8 January 2024EricMiller talk contribs 1,833 bytes +1,833 Created page with "# Prepare 10mL of media and set up cells to incubate for 1 hour in the 37C shaking incubator #* Set up for 10^8 cells which is 12.5 ul for Black WT aliquots found in the -80 freezer # After 1 hour, add phage for a total amount of 10^6 phage #* For P21 of the pre-adapted phage, the amount needed is 8.62 ul # Start the timer as soon as the phage has been added and incubate at 37C #* The best way to do this now is to place in the shaking incubator after adding phage and whe..."