Electroporation and Plating of M. xanthus transformants: Revision history

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25 June 2024

  • curprev 16:1816:18, 25 June 2024‎ Jcomstock talk contribs‎ 6,555 bytes +6,555‎ Created page with "Dialysis Prior to electroporation of the plasmid into M. xanthus, excess salts from the plasmid extraction need to be removed, as salt will interfere with the current that passes through the sample. You will float a cellulose membrane (pore size 0.025 µm) in a well containing water, allowing for the exchange of water and salt but not plasmid. 1. Fill a 60mm Petri dish 2/3 full with sterile, diH2O. You'll need one per plasmid. Before you add the membranes, ensure that..."
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