Colony PCR to confirm correct insert: Revision history

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15 June 2023

  • curprev 10:1810:18, 15 June 2023‎ Jcomstock talk contribs‎ 2,039 bytes +2,039‎ Created page with "1. Obtain 3 PCR tubes per sample, and two extra for controls. Label the tubes on the top and sides as follows: one ‘u’ for the untransformed E. coli control, one “-” for the negative control with no E. coli cells. The rest you will label in the next step. 2. Choose three patches of transformed E. coli per sample that display good growth on the LB/kan plate. Record in your notebook which patches you have chosen from, and label the rest of the PCR tubes with th..."
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