Transform competent E. coli cells: Revision history

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29 March 2023

  • curprev 12:0212:02, 29 March 2023‎ Tommy talk contribs‎ 2,149 bytes +13‎ No edit summary
  • curprev 12:0112:01, 29 March 2023‎ Tommy talk contribs‎ 2,136 bytes 0‎ No edit summary
  • curprev 12:0012:00, 29 March 2023‎ Tommy talk contribs‎ 2,136 bytes −10‎ No edit summary
  • curprev 12:0012:00, 29 March 2023‎ Tommy talk contribs‎ 2,146 bytes +2,146‎ Created page with "Transform competent E. coli cells 1.Prepare for transformation by ensuring the following: Water bath is set to 42°C Vial of SOC medium is warmed to room temperature X-gal/Kan plates are warmed to 37°C One Shot® Chemically Competent E. coli are thawed on ice Tubes of competent cells will be shaken horizontally which means cells will come into contact with the cap of the tube. To maintain good sterile technique and prevent contamination, do not touch the insi..."
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