Streptococcus CRISPR-Cas9 Editing: Revision history

16 December 2022

• curprev 12:5712:57, 16 December 2022‎ Ekhgn0 talk contribs‎ m 6,259 bytes 0‎ 6 revisions imported

6 September 2022

• curprev 12:4012:40, 6 September 2022‎ PreDec2022>EricMiller‎ 6,259 bytes +128‎ →‎Transformation Protocol with CRISPR: Adding in plasmid variant (pDSxxx)

1 September 2022

• curprev 12:4412:44, 1 September 2022‎ PreDec2022>MSmith9‎ 6,131 bytes +43‎ →‎Transformation Protocol with CRISPR: Adding in HR template
• curprev 12:4212:42, 1 September 2022‎ PreDec2022>MSmith9‎ 6,088 bytes 0‎ →‎Transformation Protocol with CRISPR: Adding in plasmid variant (pDSxxx)

31 August 2022

• curprev 21:5921:59, 31 August 2022‎ PreDec2022>MSmith9‎ 6,088 bytes +459‎ →‎Overall Workflow: Adding figure of pDSO5 plasmid
• curprev 21:5221:52, 31 August 2022‎ PreDec2022>MSmith9‎ 5,629 bytes +2,527‎ Updating this protocol to be more specific for the CRISPR- Cas 9 system and adding in HR. Additionally, adding a workflow that shows how the multiple parts work together.

15 August 2022

• curprev 08:5408:54, 15 August 2022‎ PreDec2022>EricMiller‎ 3,102 bytes +3,102‎ Created page with " == Reagents Needed== * pSD05 plasmid (P-80 in our freezer) == Designing gRNA Cut Site== # Use Benchling CRISPR guide along with the specific genome that you want to change. Find the gene what we want to disrupt, and have the CRISPR guide xxxxx # Design 8 primers: ## Two primers that, when put together, will create the proper gRNA guide RNA. There's not PCR needed for this; it is instead annealing the two primers together. Primers should be in the form XXXXXX and XXXX..."