Preparing the Plates

From Microbial Ecology and Evolution Lab Wiki
Jump to navigation Jump to search

Prepare and label your petri dishes

1. Dish should have: Date, Your name, Strain, Dilution, and Duplicate ID

2. Try to label with small handwriting around the edges to make future plaque counting easier

3. The dilution labels on the petri dishes should only correspond to the serial dilutions that you wish to plate (for example: 10^6 -> 10^8, so 3 plates), do not plate every serial dilution

4. If you are working in multiples (like triplicates), calculate total amount of plates needed, ID them with letters

5. After labeling plates, place them in an incubator to warm