PCR-based Methods

qPCR machine, portable:

• • https://www.tetracore.com/t-cor8/index.html

Note: Seems quite expensive, as it is for biosecurity. The company initially replied to emails, but no final price.

Open Source PCR and Chai Open qPCR

• • https://www.chaibio.com/openqpcr

Helpful reviews:

• https://www.boslab.org/single-post/2017/07/10/Open-qPCR-test-run
• https://www.researchgate.net/post/Has_anyone_used_the_Open_qPCR_device_made_by_Chai

Open Source PCR ($499)

• https://openpcr.org/

It looks like the people who created the open source PCR did not have the production capabilities, and so they paired up / created Chai Bio. Therefore, the open source PCR machine is no longer available to purchase. However, it led to a Kickstarter campaign for the open source qPCR machine. Chai bio sells this Open qPCR for Single channel $4,299 and Dual channel $5,999.

But, the software is open source, and the 3D CAD model is online at:

https://grabcad.com/library/chai-open-qpcr-1

~$300 qPCR machine with CAD plans:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460903/#pone.0179133.s008

Mini PCR and Gel Electrophoresis (in-field)

• https://www.minipcr.com/applications/in-the-field/ ($650)
• https://www.minipcr.com/

BIOMEME Portable Smartphone ready qPCR - ($9,950 for 3)

• https://biomeme.com/?gclid=Cj0KCQiAzKnjBRDPARIsAKxfTRCu5q5Jp2-yNgL0hpwPA5LFv1Z-G_4kfOGO7qRK2JMz8rSIN52hTkoaAg3KEALw_wcB

Loop-mediated isothermal amplification (LAMP):

• Minireview: Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects
• Simultaneous DNA amplification and detection using a pH-sensing semiconductor system
• LAMP for Human African Trypanosomiasis: A Comparative Study of Detection Formats
• Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes

qPCR machines:

• $300 PCR machine from Dr Brian Kim's group in South Flordia: Unclear if possible to change PCR temperatures. Requires a large amount of reagents (20ul) and 20ul mineral oil instead of a heated lid. Only 1 sample at a time. Matlab might be required to initialize results.
• open PCR machine was bought by Chai; it is no longer commercially made [1]. This website does have the maker instructions for it.
• Chai makes an open qPCR machine [2]. $4,300 for one channel, $6,000 for dual channel. open source code open source construction
• Biomeme has a pre-shipment travel qPCR machine with 3 channels. $9,950. [3]

LAMP detection

• Genie III Instrument Portable, robust LAMP with temperature control and dual channel fluorescence. Bluetooth and WiFi. Battery powered. 8 tube strips. $18,000