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  • Check with Eric and Jess if any microbiology media needs to be made.
  • If so, make media before 10am so that it can be autoclaved in the morning by Nicole. The process takes about an hour. Media cannot sit overnight before autoclaving.
  • Media recipes can be found on the lab wiki.
  • Cover prepared media loosely with a cap so that steam can enter the flask/bottle. Label prepared media with a piece of autoclave tape that has the lab initials (MEE), your initials, date, and media content written on it (e.g. MEE AP TSB Media 04/27/22).
  • Also, put a small tag of autoclave tape on the bottle — autoclave tape is in the first drawer beneath the small water bath, next to the sink.
  • Place bottle in the to-be autoclaved area