Making a Broth Culture from an Agar Plate: Revision history

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17 February 2023

  • curprev 08:1108:11, 17 February 2023Jcomstock talk contribs 2,789 bytes +2,789 Created page with "Making a broth culture from an agar plate To do essentially all experiments with M. xanthus, we need to make overnight broth cultures in CTTYE. This allows us to control the cell density more carefully for phenotype assays. 1. Obtain a sterile flask with an overturned sterile beaker as a lid (cabinet under the incubators). It is crucial that you use a sterile flask for this. If one is not available, talk to Jess and we can figure out a solution. 2. Working by the stat..."