Ligation of PCR product into TOPO 2.1 vector: Revision history

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6 June 2024

24 March 2023

  • curprev 10:2610:26, 24 March 2023Jcomstock talk contribs 1,415 bytes +1,415 Created page with "Before proceeding with ligation, please make sure that you have done a PCR clean-up reaction if necessary, following the Zymo DNA clean and concentrator kit. If needed [only if PCR product has been sitting in the freezer for >5 days]: Adding A’s To ensure the presence of 3’ A overhangs on the PCR product add 1 to 5 units of Taq polymerase (1ul of GoTaq) to your PCR tube. Place in PCR machine programmed to run 94oC for 3min and incubate the tube at 72°C for 10 mi..."