Generate St Curve for OD600 to Cells/mL conversion: Revision history

Jump to navigation Jump to search

Diff selection: Mark the radio buttons of the revisions to compare and hit enter or the button at the bottom.
Legend: (cur) = difference with latest revision, (prev) = difference with preceding revision, m = minor edit.

26 May 2023

  • curprev 09:3009:30, 26 May 2023Jcomstock talk contribs 1,766 bytes +1,766 Created page with "1. Obtain O/N M. xanthus cultures at different densities (ideally relatively equally spaced to achieve close to 0.2, 0.4, 0.6, and 0.8 OD600. 2. Measure the absorbance of these samples and convert values to OD600 by factoring in the path length. Record these values. 3. Melt down CTTSA in the microwave, using caution not to boil it over. Heat in increments of 30 seconds once it starts melting. 4. Aliquot 4mL of CTTSA into as many tubes as is needed for your experime..."