Commonly Used Plasmids

pUC18

Standard, high-copy number E. coli plasmid with ampicillin (AMP) resistance. Multiple cloning site in a lacZ gene. addgene.org

pDHC29

Standard, high-copy number E. coli plasmid with chlorophenicol (CAM) resistance; additionally, we have prepared tubes for blunt-end cloning. lacZ gene is present. addgene.org

pJPK13

Standard, high-copy number E. coli plasmid with kanamycin (KAN) resistance. Multiple cloning site in a lacZ gene. addgene.org

pBR322

Standard, low-copy number E. coli plasmid with dual ampicillin (AMP) and tetracycline (TET) resistance. No lacZ gene. addgene.org

pACYC184

Standard, high-copy E. coli plasmid with dual chloramphenicol (CAM) and tetracycline (TET) resistance. No lacZ gene. addgene.org

pNZ8048

Low-copy number L. lactis plasmid. Chloramphenicol (CAM) resistance. Promoter controlled by nisin (which we do not use). Good for putting into Gram-positive bacteria. No lacZ gene.

pET 1GFP

Full name is pET His6 GFP TEV LIC cloning vector (1GFP). E.coli plasmid, but should be put into BL21 cells for maximum protein synthesis. Histidine tag attached to GFP attached to a TEV protease site attached to another histidine tag. Useful for creating GFP-linked proteins. Kanamycin resistance. No lacZ gene. addgenes.org