Creating Lac- E. coli Mutants

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Goal

To create Lac negative E. coli mutants for student/faculty use.

Pre-Protocol Questions

1. Do you know how to properly streak a strain onto a plate using an inoculating loop?

2. Do you know how to pick a single colony from a plate?

3. Do you have liquid LB media? sterile Erlenmeyer flasks? milliQ water? virkon solution? any other materials you will need for this protocol?

4. Do you know how to use the Plasmid Miniprep Kit? the NanoDrop Machine?

Protocol